Rat B7-1/CD80 ELISA Kit

96T/2480

Activation B7 1 antigen; B7; B7 1; B7-1; BB1; CD28LG; CD28LG1; CD80; CD80 molecule; CTLA 4 counter receptor B7.1; LAB7

Expression system for standard: NS0; Immunogen sequence: I39-Q248

There is no detectable cross-reactivity with other relevant proteins.

<2pg/ml

62.5pg/ml-4000pg/ml

CD80

CD80 molecule

O35187

Rat B7-1/CD80 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0709)

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

15-20分钟

Catalog number	Description	Quantity
EK0709-CAP	96-well plate precoated with anti- rat CD80 antibody	1
EK0709-ST	Lyophilized recombinant rat CD80 standard	10ng/tube×2
EK0709-DA	Biotinylated anti- rat CD80 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	154	448	1666	165	472	1688
Standard deviation	10.78	21.5	89.96	13.69	29.26	113.09
CV(%)	7%	4.8%	5.4%	8.3%	6.2%	6.7%

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.019	0.060	0.092	0.178	0.406	0.799	1.611	2.283

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants and Serum