Rat B7-2/CD86 ELISA Kit

96T/2480

Activation B7 2 antigen; B7 2; B70; BU63; CD28LG2; CD86; CD86 molecule; FUN 1; LAB72

Expression system for standard: NS0; Immunogen sequence: V29-I250

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

62.5pg/ml-4000pg/ml

CD86

CD86 molecule

Q62810

Rat B7-2/CD86 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0712)

Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

25-30分钟

Catalog number	Description	Quantity
EK0712-CAP	96-well plate precoated with anti- rat CD86 antibody	1
EK0712-ST	Lyophilized recombinant rat CD86 standard	10ng/tube×2
EK0712-DA	Biotinylated anti- rat CD86 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	179	612	1878	193	658	1765
Standard deviation	10.2	44.67	110.8	13.89	51.98	112.96
CV(%)	5.7%	7.3%	5.9%	7.2%	7.9%	6.4%

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.058	0.135	0.208	0.365	0.614	0.986	1.727	2.367

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum