Human 2B4/CD24 ELISA Kit

96T/2480

2B4; CD244; h2B4; NAIL; NKR2B4; SLAMF4

NSO, C22-R221

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

15.6 pg/ml - 1000 pg/ml

CD244

CD244 molecule, natural killer cell receptor 2B4

Q9BZW8

Human 2B4/CD24 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0926)

CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.

25-30分钟

Catalog number	Description	Quantity
EK0926-CAP	96-well plate precoated with anti- human CD244 antibody	1
EK0926-ST	Lyophilized recombinant human CD244 standard	10ng/tube×2
EK0926-DA	Biotinylated anti- human CD244 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	28	81	453	30	112	469
Standard deviation	1.34	4.62	20.84	1.53	6.61	30.02
CV(%)	4.8	5.7	4.6	5.1	5.9	6.4

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.025	0.199	0.292	0.464	0.934	1.510	2.026	2.547

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h