Human ADAMTS13 ELISA Kit

96T/2480

ADAM TS 13; ADAM TS13; ADAMTS 13; ADAMTS13; C9orf8; DKFZp434C2322; FLJ42993; TTP; vWF cleaving protease; vWF CP; VWFCP

CHO, Q34-W688

There is no detectable cross-reactivity with other relevant proteins.

<20 pg/ml

0.78 ng/ml - 50 ng/ml

ADAMTS13

ADAM metallopeptidase with thrombospondin type 1 motif, 13

Q76LX8

Human ADAMTS13 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0927)

ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

25-30分钟

Catalog number	Description	Quantity
EK0927-CAP	96-well plate precoated with anti- human ADAMTS13 antibody	1
EK0927-ST	Lyophilized recombinant human ADAMTS13 standard	50ng/tube×2
EK0927-DA	Biotinylated anti- human ADAMTS13 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1395	7236	20816	1322	6617	20301
Standard deviation	104.62	289.44	1165.69	104.43	291.14	1197.75
CV(%)	7.5	4	5.6	7.9	4.4	5.9

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.051	0.101	0.134\0.243	0.439	0.829	1.482	2.472

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h