Human ADAM12 ELISA Kit

96T/2480

ADAM 12; ADAM12; MCMP; MCMPMltna; Meltrin alpha; MLTN; MLTNA

CHO, R29-S513

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

156 pg/ml - 10000 pg/ml

ADAM12

ADAM metallopeptidase domain 12

O43184

Human ADAM12 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0934)

ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM(a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

15-20分钟

Catalog number	Description	Quantity
EK0934-CAP	96-well plate precoated with anti- human ADAM12 antibody	1
EK0934-ST	Lyophilized recombinant human ADAM12 standard	10ng/tube×2
EK0934-DA	Biotinylated anti- human ADAM12 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	285	1490	4755	259	1498	4763
Standard deviation	16.81	75.99	256.77	19.68	98.86	338.17
CV(%)	5.9	5.1	5.4	7.6	6.6	7.1

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.027	0.084	0.138	0.264	0.491	0.831	1.340	2.170

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin) and Urine

3.5h