Human Adipsin/CFD ELISA Kit

96T/2480

ADIPSIN; ADN; C3 convertase activator; CFD; Complement factor D; complement factor D (adipsin); DF; PFD; Properdin factor D

NSO, I26-A253

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

312 pg/ml - 20000 pg/ml

CFD

complement factor D (adipsin)

P00746

Human Adipsin/CFD ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1199)

Complement factor D(CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

25-30分钟

Catalog number	Description	Quantity
EK1199-CAP	96-well plate precoated with anti- human CFD antibody	1
EK1199-ST	Lyophilized recombinant human CFD standard	20ng/tube×2
EK1199-DA	Biotinylated anti- human CFD antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	787	2378	9095	804	2193	8576
Standard deviation	44.07	149.81	436.56	49.84	144.73	471.68
CV(%)	5.6	6.3	4.8	6.2	6.6	5.5

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.009	0.093	0.182	0.358	0.701	1.156	1.822	2.355

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine

3.5h