Rat BTC ELISA Kit

说明书

EK1297

96T ￥2480
货期：现货

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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Rat BTC ELISA Kit

规格/价格

96T/2480

指标别名

betacellulin; BTC; Probetacellulin

免疫原

Expression system for standard: E.coli; Immunogen sequence: D32-Y111

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10pg/ml

检测范围

7.8pg/ml-500pg/ml

基因名称

BTC

基因名全称

betacellulin

Uniprot ID

Q9JJM4

文献引用格式

Rat BTC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1297)

背景资料

Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK1297-CAP	96-well plate precoated with anti- rat BTC antibody	1
EK1297-ST	Lyophilized recombinant rat BTC standard	10ng/tube×2
EK1297-DA	Biotinylated anti- rat BTC antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	15	51	280	16	55	280
Standard deviation	0.6	3.72	14	0.92	4.29	17.64
CV(%)	4%	7.3%	5%	5.8%	7.8%	6.3%

典型数据示例

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.100	0.118	0.128	0.171	0.253	0.421	0.837	1.801

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

应用范围

ELISA

检验物种

rat

检测方法

酶联免疫双抗夹心法

样本类型

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

实验方案>

实验步骤

Rat BTC ELISA Kit

EK1297

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验方案>

引用文献(0)>

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