Rat Adiponectin/ADIPOQ ELISA Kit

96T/2480

ACDC; ACRP30; adiponectin; ADIPOQ; ADIPQTL1; ADPN; APM 1; APM1; GBP28; Gelatin binding protein

Expression system for standard: E.coli; Immunogen sequence: I19-N244

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

156pg/ml-10000pg/ml

ADIPOQ

adiponectin, C1Q and collagen domain containing

Q8K3R4

Rat Adiponectin/ADIPOQ ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1327)

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

25-30分钟

Catalog number	Description	Quantity
EK1327-CAP	96-well plate precoated with anti- rat Adiponectin antibody	1
EK1327-ST	Lyophilized recombinant rat Adiponectin standard	10ng/tube×2
EK1327-DA	Biotinylated anti- rat Adiponectin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	292	1705	4269	265	1626	4075
Standard deviation	16.93	126.17	170.76	19.61	133.33	167.07
CV(%)	5.8%	7.4%	4%	7.4%	8.2%	4.1%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.004	0.086	0.164	0.305	0.588	0.961	1.511	1.937

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Serum, Plasma (heparin, EDTA)and Urine