Human ADAMTS1 ELISA Kit

96T/2480

ADAM TS 1; ADAM TS1; ADAMTS 1; ADAMTS1; C3 C5; KIAA1346; METH 1; METH1

NSO, F253-A734

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

93.8 pg/ml - 6000 pg/ml

ADAMTS1

ADAM metallopeptidase with thrombospondin type 1 motif, 1

Q9UHI8

Human ADAMTS1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1340)

ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

20-25分钟

Catalog number	Description	Quantity
EK1340-CAP	96-well plate precoated with anti- human ADAMTS1 antibody	1
EK1340-ST	Lyophilized recombinant human ADAMTS1 standard	10ng/tube×2
EK1340-DA	Biotinylated anti- human ADAMTS1 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	145	681	2886	132	732	2843
Standard deviation	8.84	37.45	207.79	10.56	51.97	213.22
CV(%)	6.1	5.5	7.2	8	7.1	7.5

Concentration(pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.026	0.096	0.160	0.278	0.513	0.920	1.599	2.476

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin)

3.5h