Human ADAMTS4 ELISA Kit

96T/2480

ADAM TS 4; ADAM TS4; ADAMTS 2; ADAMTS 4; ADAMTS4; ADMP 1; Aggrecanase 1; KIAA0688

CHO, F213-C685

There is no detectable cross-reactivity with other relevant proteins.

<20 pg/ml

625 pg/ml - 40000 pg/ml

ADAMTS4

ADAM metallopeptidase with thrombospondin type 1 motif, 4

O75173

Human ADAMTS4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1372)

ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

15-20分钟

Catalog number	Description	Quantity
EK1372-CAP	96-well plate precoated with anti- human ADAMTS4 antibody	1
EK1372-ST	Lyophilized recombinant human ADAMTS4 standard	40ng/tube×2
EK1372-DA	Biotinylated anti- human ADAMTS4 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1127	4290	29063	1210	4672	29313
Standard deviation	46.2	184.47	1627.52	71.39	200.89	2081.22
CV(%)	4.1	4.3	5.6	5.9	4.3	7.1

Concentration(pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.025	0.106	0.182	0.301	0.535	1.005	1.655	2.265

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h