Human ADAM15 ELISA Kit

96T/2480

ADAM 15; ADAM15; MDC 15; MDC15; Metargidin

NSO, D207-T696

There is no detectable cross-reactivity with other relevant proteins.

<12 pg/ml

62.5 pg/ml - 4000 pg/ml

ADAM15

ADAM metallopeptidase domain 15

Q13444

Human ADAM15 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1484)

Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

15-20分钟

Catalog number	Description	Quantity
EK1484-CAP	96-well plate precoated with anti- human ADAM15 antibody	1
EK1484-ST	Lyophilized recombinant human ADAM15 standard	10ng/tube×2
EK1484-DA	Biotinylated anti- human ADAM15 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	112	674	1454	110	704	1511
Standard deviation	5.04	53.24	59.61	7.04	64.06	64.97
CV(%)	4.5	7.9	4.1	6.4	9.1	4.3

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.061	0.083	0.109	0.185	0.404	0.664	1.373	2.079

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h