Human Afamin/AFM ELISA Kit

说明书

EK1487

96T ￥2480
货期：现货

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产品简介>

产品名称

Human Afamin/AFM ELISA Kit

规格/价格

96T/2480

指标别名

afamin; AFM; ALB2; ALBA; ALF; Alpha Alb; Alpha albumin

免疫原

NSO, L22-N599

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10 pg/ml

检测范围

156pg/ml-10000pg/ml

基因名称

AFM

基因名全称

afamin

Uniprot ID

P43652

文献引用格式

Human Afamin/AFM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1487)

背景资料

Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK1487-CAP	96-well plate precoated with anti- human Afamin antibody	1
EK1487-ST	Lyophilized recombinant human Afamin standard	2ng/tube×2
EK1487-DA	Biotinylated anti- human Afamin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	54	287	838	49	308	783
Standard deviation	2.75	18.94	61.17	2.84	25.87	60.29
CV(%)	5.1	6.6	7.3	5.8	8.4	7.7

典型数据示例

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.030	0.149	0.251	0.480	0.751	1.261	1.806	2.089

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

应用范围

ELISA

检验物种

human

检测方法

酶联免疫双抗夹心法

样本类型

Cell Culture Supernatants, Serum and Urine

实验时长

3.5h

实验方案>

实验步骤

Human Afamin/AFM ELISA Kit

EK1487

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

实验方案>

引用文献(0)>

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