Rat BIGH3/TGFBI ELISA Kit

96T/2480

Beta ig h3; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; Kerato epithelin; LCD1; RGD CAP; TGFBI; TGFBI / BIGH3

Expression system for standard: NS0; Immunogen sequence: G24-G685

There is no detectable cross-reactivity with other relevant proteins.

<15pg/ml

156pg/ml-10000pg/ml

TGFBI

transforming growth factor, beta-induced, 68kDa

Q15582

Rat BIGH3/TGFBI ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1571)

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

15-20分钟

Catalog number	Description	Quantity
EK1571-CAP	96-well plate precoated with anti- rat beta IG-H3antibody	1
EK1571-ST	Lyophilized recombinant rat beta IG-H3 standard	10ng/tube×2
EK1571-DA	Biotinylated anti- rat beta IG-H3 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	308	1355	5623	336	1296	5160
Standard deviation	20.63	73.17	314.88	24.19	84.24	309.6
CV(%)	6.7%	5.4%	5.6%	7.2%	6.5%	6%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.010	0.031	0.064	0.137	0.376	0.922	1.879	2.599

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)