Rat ALCAM ELISA Kit

96T/2480

ALCAM; CD166; CD166 antigen; MEMD

Expression system for standard: NS0; Immunogen sequence: W28-K527

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

78pg/ml-5000pg/ml

ALCAM

activated leukocyte cell adhesion molecule

O35112

Rat ALCAM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1572)

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

25-30分钟

Catalog number	Description	Quantity
EK1572-CAP	96-well plate precoated with anti- rat ALCAM antibody	1
EK1572-ST	Lyophilized recombinant rat ALCAM standard	10ng/tube×2
EK1572-DA	Biotinylated anti- rat ALCAM antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	123	659	2375	124	634	2509
Standard deviation	6.39	50.74	187.62	7.93	54.52	225.81
CV(%)	5.2%	7.7%	7.9%	6.4%	8.6%	9%

Concentration(pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.004	0.064	0.118	0.215	0.450	0.798	1.578	2.317

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)