Rat BMP9/GDF2 ELISA Kit

96T/2480

BMP 9; BMP9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2

Expression system for standard: CHO; Immunogen sequence: S320-R430

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

15.6pg/ml-1000pg/ml

GDF2

growth differentiation factor 2

M0RB87

Rat BMP9/GDF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1611)

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

25-30分钟

Catalog number	Description	Quantity
EK1611-CAP	96-well plate precoated with anti- rat BMP-9 antibody	1
EK1611-ST	Lyophilized recombinant rat BMP-9 standard	10ng/tube×2
EK1611-DA	Biotinylated anti- rat BMP-9 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	36	187	414	35	192	435
Standard deviation	2.12	11.78	23.59	2.69	13.63	25.66
CV(%)	5.9%	6.3%	5.7%	7.7%	7.1%	5.9%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.073	0.133	0.189	0.300	0.503	0.944	1.524	2.369

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)