Rat Agrin/AGRN ELISA Kit

96T/2800

AGRIN; CMS8; CMSPPD

Expression system for standard: NS0; Immunogen sequence: A1153-P1959

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

31.2pg/ml-2000pg/ml

Agrn

agrin

P25304

Rat Agrin/AGRN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1699)

Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the AGRN gene.This protein has nine domains homologous to protease inhibitors.It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.

25-30分钟

Catalog number	Description	Quantity
EK1699-CAP	96-well plate precoated with anti- rat Agrin antibody	1
EK1699-ST	Lyophilized recombinant rat Agrin standard	10ng/tubex2
EK1699-DA	Biotinylated anti- rat Agrin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	57	219	767	60	223	776
Standard deviation	2.39	10.73	39.1	3.36	14.27	50.1
CV(%)	4.2%	4.9%	5.1%	5.6%	6.4%	7.1%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.079	0.156	0.211	0.336	0.565	0.991	1.741	2.376

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)