Human Acetylcholinesterase/ACHE ELISA Kit

96T/2480

Acetylcholinesterase; ACHE; ARACHE; N ACHE; YT

CHO, E32-L614

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

62.5 pg/ml - 4000 pg/ml

ACHE

acetylcholinesterase (Cartwright blood group)

P22303

Human Acetylcholinesterase/ACHE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2137)

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion.

15-20分钟

Catalog number	Description	Quantity
EK2137-CAP	96-well plate precoated with anti- human ADAMTS13 antibody	1
EK2137-ST	Lyophilized recombinant human ADAMTS13 standard	50ng/tube×2
EK2137-DA	Biotinylated anti- human ADAMTS13 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	46	163	278	52	178	305
Standard deviation	2.07	7.66	9.17	2.7	10.32	15.86
CV(%)	4.5	4.7	3.3	5.2	5.8	5.2

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.060	0.152	0.233	0.358	0.594	0.883	1.502	2.135

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

Acetylcholinesterase

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h