产品名称
Human ACP5 ELISA Kit
指标别名
ACP5; Tartrate resistant acid ATPase; TR AP; TRACP; TRAP; TrATPase; Type 5 acid phosphatase
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
acid phosphatase 5, tartrate resistant
文献引用格式
Human ACP5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2138)
背景资料
Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK2138-CAP | 96-well plate precoated with anti- human ADAMTS13 antibody | 1 |
EK2138-ST | Lyophilized recombinant human ADAMTS13 standard | 50ng/tube×2 |
EK2138-DA | Biotinylated anti- human ADAMTS13 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 352 | 1277 | 4565 | 385 | 1365 | 4632 |
Standard deviation | 19.36 | 71.51 | 146.08 | 23.49 | 94.19 | 213.07 |
CV(%) | 5.5 | 5.6 | 3.2 | 6.1 | 6.9 | 4.6 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.4 | 125 | 250 | 500 | 1,000 | 2,000 |
O.D. | 0.041 | 0.085 | 0.191 | 0.391 | 0.864 | 1.230 | 1.863 | 2.368 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
蛋白名全称
Tartrate-resistant acid phosphatase type 5
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)