Human CD32b/c EZ-Set™ ELISA Kit (DIY Antibody Pairs)

5 plates(kit)/3980

CD32; CD32B; CDw32; Fc gamma RII b; Fc gamma RIIb; FCG2; FCGR2; FCGR2B; FcRII b; IGFR2; IgG Fc receptor II b; IgG Fc receptor II-c; CDw32; Fc-gamma RII-c; Fc-gamma-RIIc; FcRII-c; FCGR2C; IGFR2

Expression system for standard: NS0; Immunogen sequence: A46-P217

There is no detectable cross-reactivity with other relevant proteins.

46.9 pg/ml - 3,000 pg/ml

FCGR2B/FCGR2C

Fc fragment of IgG receptor Iib

P31994/P31995

Human CD32b/c EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ1312)

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ1312-CA	Rabbit anti- human FCGR2B/C polyclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ1312-DA	Biotinylated goat anti- human FCGR2B/C polyclonal antibody (Detection Antibody)	500 µl, 6 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EZ1312-ST	Lyophilized recombinant human FCGR2B/C standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.007	0.124	0.199	0.255	0.541	0.813	1.408	2.266

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。

human

Low affinity immunoglobulin gamma Fc region receptor II-b

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)