Anti-Trimethyl-Histone H3 (Lys79)/H3K79me3 Antibody (Clone#RM157)

Rabbit monoclonal antibody

说明书

M06819-19

100μg ￥6192
货期： 3-4周

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Figure 1. Western Blotting result
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM157 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 79 (K79me3) in HeLa cells.
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Figure 2. Specificity Test result
RM157 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.
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Figure 3. Dot Blot result
A Peptide dotblot shows RM157 only reacts to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity with nonmodified Lysine 79 (H3K79Ctrl), monomethylated Lysine 79 (K79me1) or dimethylated Lysine 79 (K79me2).
all(3)

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产品名称

Anti-Trimethyl-Histone H3 (Lys79)/H3K79me3 Antibody (Clone#RM157)

规格/价格

100μg/6192

指标别称

H3 histone; family 3A; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; Histone H3.3

产品类型

Monoclonal

检验物种

human, Vertebrates

应用范围

WB, ELISA, Luminex

基因名称

H3K79me3

克隆号

RM157

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys79)

计算分子量

15 kDa

成分

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

纯化方式

Protein A affinity purified from an animal origin-free culture supernatant

浓度

1 mg/mL

保存条件

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

文献引用格式

Trimethyl-Histone H3 (Lys79)/H3K79me3 Antibody (Clone#RM157) (Boster Biological Technology, Wuhan, China. Catalog#M06819-19)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Histone H3.3

实验方案>

实验步骤

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