Anti-Dimethyl Histone H3 (Lys79)/H3K79me2 Antibody (Clone#RM181)

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  Figure 1. Western Blotting result
  Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.

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  Figure 2. Specificity Test result
  RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.

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  Figure 3. IHC result
  Immunohistochemistry staining of HepG2 cell using anti-Dimethyl-Histone H3 (Lys79) antibody, RM181.

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  Figure 4. ChIP result
  ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5ug). Real-time PCR was performed using primers specific to the gene indicated.