Anti-(Mouse) Claudin 6/CLDN6 Antibody (C-term)

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  Western blot analysis of lysate from mouse kidney tissue lysate, using Cldn6 Antibody (C-term). M08182-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

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  Overlay histogram showing colon26 cells stained with M08182-1 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08182-1, 1:25 dilution) for 60 min at 37oC. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.