Anti-5 Lipoxygenase/ALOX5 Antibody

50μl/1180 100μl/1960 150μl/2780

5 Lipoxygenase; 5 LO; 5 LOX; 5LPG; ALOX5; LOG5

Polyclonal

human, mouse, rat

IHC,WB

ALOX5

Rabbit

IgG

E.coli-derived human ALOX5 recombinant protein (Position: A120-R483). Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.

78 kDa

78 kDa

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

Immunogen affinity purified.

500 ug/ml

Liquid

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.

P09917

5 Lipoxygenase/ALOX5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0008)

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

arachidonate 5-lipoxygenase

Arachidonate 5-lipoxygenase

Western blot (WB):	1:500-2000
Immunohistochemistry (IHC):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.