Anti-ZO-1/TJP1 Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of anti- TJP1 antibody (PB9234). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human PC-3 whole cell lysates,
  Lane 2: human Caco-2 whole cell lysates,
  Lane 3: human Colo320 whole cell lysates,
  Lane 4: rat testis tissue lysates,
  Lane 5: mouse testis tissue lysates.
  Use rabbit anti- TJP1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TJP1 at approximately 220KD. The expected band size for TJP1 is at 185KD.

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  Figure 2. IHC analysis of TJP1 using anti-TJP1 antibody (PB9234).TJP1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TJP1 Antibody (PB9234) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. ICC analysis using anti-TJP1/ZO-1 antibody (PB9234) was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).

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  Figure 4. Flow cytometry analysis of K562 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).