Anti-BRCA1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF

A00005

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of BRCA1 using anti-BRCA1 antibody (A00005). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (A00005) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BRCA1 at approximately 290 kDa. The expected band size for BRCA1 is at 208 kDa.
all(2)
ICC/IF analysis of BRCA1 using anti-BRCA1 antibody (A00005) and anti-Beta Tubulin antibody (M01857-3).
BRCA1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-BRCA1 Antibody (A00005) at a dilution of 1:100. Fluoro 488 Conjugated AffiniPure Donkey Anti-rabbit IgG (H+L) (green)(Catalog#BA1146) and Fluoro594-conjugated Goat Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1141) were used as secondary antibody.
all(2)

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产品简介>

产品名称

Anti-BRCA1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BRCA1; BRCAI; BRCC1; breast cancer 1; early onset; BROVCA1; IRIS; PSCP; RING finger protein 53; RNF53

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

BRCA1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human BRCA1 recombinant protein (Position: T1681-E1781). Human BRCA1 shares 73.3% and 74.3% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively.

计算分子量

208 kDa

实际分子量

290 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

BRCA1, mapped to 17q21.3, is also known as BRCC1. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.

Uniprot ID

P38398

RRID

AB_3080875

文献引用格式

BRCA1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00005)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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