Anti-BRCA1 Antibody (Clone#OTI5D10)

Mouse monoclonal antibody

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筛选器： All WB IHC

M00005-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRCA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRCA1(Cat# M00005-1).
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00005-1) (1:200)
all(10)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-BRCA1 Antibody (Clone#OTI5D10)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BRCA1; BRCAI; BRCC1; breast cancer 1; early onset; BROVCA1; IRIS; PSCP; RING finger protein 53; RNF53

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC

基因名称

BRCA1

克隆号

OTI5D10

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 1151-1473 of human BRCA1 (NP_009225) produced in E.coli.

计算分子量

208 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

BRCA1, mapped to 17q21.3, is also known as BRCC1. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.

Uniprot ID

P38398

文献引用格式

BRCA1 Antibody (Clone#OTI5D10) (Boster Biological Technology, Wuhan, China. Catalog#M00005-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

BRCA1 DNA repair associated

蛋白名全称

Breast cancer type 1 susceptibility protein

实验方案>

实验步骤

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