Anti-BRCA1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM

PB9015

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- BRCA1 antibody (PB9015). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human Raji whole cell lysates.
Use rabbit anti- BRCA1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for BRCA1 at approximately 290KD. The expected band size for BRCA1 is at 208KD.
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IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015).
BRCA1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-BRCA1 Antibody (PB9015) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015).
BRCA1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-BRCA1 Antibody (PB9015) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015).
BRCA1 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-BRCA1 Antibody (PB9015) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015).
BRCA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-BRCA1 Antibody (PB9015) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015).
BRCA1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-BRCA1 Antibody (PB9015) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015).
BRCA1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-BRCA1 Antibody (PB9015) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
Flow Cytometry analysis of HepG2 cells using anti-BRCA1 antibody (PB9015).
Overlay histogram showing HepG2 cells stained with PB9015 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRCA1 Antibody (PB9015) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-BRCA1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BRCA1; BRCAI; BRCC1; breast cancer 1; early onset; BROVCA1; IRIS; PSCP; RING finger protein 53; RNF53

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM

基因名称

BRCA1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively.

计算分子量

208 kDa

实际分子量

290 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.

Uniprot ID

P38398

RRID

AB_3082969

文献引用格式

BRCA1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9015)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

breast cancer 1, early onset

蛋白名全称

Breast cancer type 1 susceptibility protein

实验方案>

实验步骤

引用文献(0)>

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