Anti-Caspase 1(p20)/CASP1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00048-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Caspase 1(p20)/CASP1 antibody (A00048-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-2 whole cell lysates,
Lane 2: human U937 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 1(p20)/CASP1 antigen affinity purified polyclonal antibody (A00048-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 1(p20)/CASP1 at approximately 47 kDa. The expected band size for Caspase 1(p20)/CASP1 is at 47 kDa.
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产品名称

Anti-Caspase 1(p20)/CASP1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CASP 1; CASP1; Caspase 1; Caspase1; ICE; IL 1 beta converting enzyme; IL 1BC; IL1BC; IL1BCE; p10; p20; P45

产品类型

Polyclonal

检验物种

human

应用范围

WB, ELISA

基因名称

CASP1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Caspase 1(p20)/CASP1 recombinant protein (Position: N132-H404). Human CASP1 shares 67.3% and 69.2% amino acid (aa) sequence identity with mouse and rat CASP1, respectively.

计算分子量

45 kDa

实际分子量

45 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Caspase 1, apoptosis-related cysteine protease, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. Caspase 1 was purified ICE from the cytosol of the THP. human monocytic cell line and found that the active protease was made up of 2 peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

Uniprot ID

P29466

Gene ID

834

文献引用格式

Caspase 1(p20)/CASP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00048-1)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

caspase 1

蛋白名全称

Caspase-1

实验方案>

实验步骤

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