Anti-Caspase 1/CASP1 (p20) Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PA1440

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Caspase 1/CASP1 (p20) using anti-Caspase 1/CASP1 (p20) antibody (PA1440). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: JURKAT whole cell lysates ,
Lane 2: RAJI whole cell lysates,
Lane 3: CEM whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 1/CASP1 (p20) antigen affinity purified polyclonal antibody (PA1440) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 1/CASP1 (p20) at approximately 45 kDa. The expected band size for Caspase 1/CASP1 (p20) is at 45 kDa.
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IHC analysis of Caspase 1/CASP1 (p20) using anti-Caspase 1/CASP1 (p20) antibody (PA1440).
Caspase 1/CASP1 (p20) was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-Caspase 1/CASP1 (p20) Antibody (PA1440) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品名称

Anti-Caspase 1/CASP1 (p20) Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CASP 1; CASP1; Caspase 1; Caspase1; ICE; IL 1 beta converting enzyme; IL 1BC; IL1BC; IL1BCE; p10; p20; P45

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

CASP1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human CASP1.

计算分子量

45 kDa

实际分子量

45 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Caspase 1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

Uniprot ID

P29466

RRID

AB_3082665

文献引用格式

Caspase 1/CASP1 (p20) Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA1440)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

caspase 1, apoptosis-related cysteine peptidase

蛋白名全称

Caspase-1(CASP-1)

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