Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat heart tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (A00362-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ASC/TMS1/PYCARD at approximately 22 kDa. The expected band size for ASC/TMS1/PYCARD is at 21 kDa.
IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-4).
ASC/TMS1/PYCARD was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of Raw264.7 cells using anti-ASC/TMS1/PYCARD antibody (A00362-4).
Overlay histogram showing Raw264.7 cells stained with A00362-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat heart tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (A00362-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ASC/TMS1/PYCARD at approximately 22 kDa. The expected band size for ASC/TMS1/PYCARD is at 21 kDa.
IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-4).
ASC/TMS1/PYCARD was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of Raw264.7 cells using anti-ASC/TMS1/PYCARD antibody (A00362-4).
Overlay histogram showing Raw264.7 cells stained with A00362-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat heart tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (A00362-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ASC/TMS1/PYCARD at approximately 22 kDa. The expected band size for ASC/TMS1/PYCARD is at 21 kDa.
IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-4).
ASC/TMS1/PYCARD was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of Raw264.7 cells using anti-ASC/TMS1/PYCARD antibody (A00362-4).
Overlay histogram showing Raw264.7 cells stained with A00362-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
