Anti-ASC/TMS1/PYCARD Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF FCM ELISA

A00362-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ASC/TMS1/Pycard antibody (A00362-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ASC/TMS1/Pycard antigen affinity purified polyclonal antibody (A00362-5) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ASC/TMS1/Pycard at approximately 22 kDa. The expected band size for ASC/TMS1/Pycard is at 22 kDa.
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IF analysis of ASC/TMS1/Pycard using anti-ASC/TMS1/Pycard antibody (A00362-5) and anti-Tubulin alpha antibody (M03989-3).
ASC/TMS1/Pycard was detected in an immunocytochemical section of RM1 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
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Flow Cytometry analysis of RAW264.7 cells using anti-ASC/TMS1/Pycard antibody (A00362-5).
Overlay histogram showing RAW264.7 cells stained with A00362-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/Pycard Antibody (A00362-5, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介>

产品名称

Anti-ASC/TMS1/PYCARD Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ASC; CARD5; hASC; PYCARD; PYD and CARD domain containing; TMS; TMS 1; TMS1

产品类型

Polyclonal

检验物种

mouse

应用范围

WB, ICC/IF, FCM, ELISA

基因名称

Pycard

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse ASC/TMS1/Pycard recombinant protein (Position: K24-S193).

计算分子量

22 kDa

实际分子量

22 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

Q9EPB4

Gene ID

66824

文献引用格式

ASC/TMS1/PYCARD Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00362-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

PYD and CARD domain containing

蛋白名全称

Apoptosis-associated speck-like protein containing a CARD

实验方案>

实验步骤

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