Anti-ASC/TMS1/PYCARD Antibody (Clone#OTI1A2)

Mouse monoclonal antibody

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筛选器： All WB FCM

M00362-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PYCARD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCARD antibody, and then analyzed by flow cytometry.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCARD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCARD.
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产品名称

Anti-ASC/TMS1/PYCARD Antibody (Clone#OTI1A2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ASC; CARD5; hASC; PYCARD; PYD and CARD domain containing; TMS; TMS 1; TMS1

产品类型

Monoclonal

检验物种

human

应用范围

WB, FCM

基因名称

PYCARD

克隆号

OTI1A2

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human PYCARD (NP_037390) produced in E.coli.

实际分子量

21.4 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot ID

Q9EPB4

文献引用格式

ASC/TMS1/PYCARD Antibody (Clone#OTI1A2) (Boster Biological Technology, Wuhan, China. Catalog#M00362-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

PYD and CARD domain containing

蛋白名全称

Apoptosis-associated speck-like protein containing a CARD

实验方案>

实验步骤

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