Anti-ATF2 Antibody

筛选器： All WB FCM ELISA

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  Figure 1. Western blot analysis of ATF2 using anti-ATF2 antibody (A00916-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: HepG2 whole cell lysates,
  Lane 3: K562 whole cell lysates,
  Lane 4: U-87MG whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat thymus tissue lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse thymus tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody (A00916-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF2 at approximately 65-75 kDa. The expected band size for ATF2 is at 55 kDa.

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  Figure 2. Flow Cytometry analysis of Hela cells using anti-ATF2 antibody (A00916-2).
  Overlay histogram showing Hela cells stained with A00916-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF2 Antibody (A00916-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 1. Western blot analysis of ATF2 using anti-ATF2 antibody (A00916-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: HepG2 whole cell lysates,
  Lane 3: K562 whole cell lysates,
  Lane 4: U-87MG whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat thymus tissue lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse thymus tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody (A00916-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF2 at approximately 65-75 kDa. The expected band size for ATF2 is at 55 kDa.

• 

  Figure 2. Flow Cytometry analysis of Hela cells using anti-ATF2 antibody (A00916-2).
  Overlay histogram showing Hela cells stained with A00916-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF2 Antibody (A00916-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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