Figure 1. Western blot analysis of anti-ATF2 antibody (BM3943). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HUVEC whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: human SiHa whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates,
Lane 8: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF2 antigen affinity purified monoclonal antibody (BM3943) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF2 at approximately 70 kDa. The expected band size for ATF2 is at 55 kDa.
Figure 2. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Immunofluorescent analysis of Hela cells, using ATF2 Antibody .
all(7) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:30 |
Figure 1. Western blot analysis of anti-ATF2 antibody (BM3943). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HUVEC whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: human SiHa whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates,
Lane 8: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF2 antigen affinity purified monoclonal antibody (BM3943) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF2 at approximately 70 kDa. The expected band size for ATF2 is at 55 kDa.
Figure 2. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Immunofluorescent analysis of Hela cells, using ATF2 Antibody .
Figure 1. Western blot analysis of anti-ATF2 antibody (BM3943). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HUVEC whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: human SiHa whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates,
Lane 8: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF2 antigen affinity purified monoclonal antibody (BM3943) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF2 at approximately 70 kDa. The expected band size for ATF2 is at 55 kDa.
Figure 2. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of ATF2 using anti-ATF2 antibody (BM3943) .
ATF2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ATF2 Antibody (BM3943) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Immunofluorescent analysis of Hela cells, using ATF2 Antibody .
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