Anti-ATF2 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

BA0653

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of ATF2 using anti-ATF2 antibody (BA0653). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: HEPG2 whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: SH-SY5Y whole cell lysates,
Lane 5: U-87MG whole cell lysates,
Lane 6: A549 whole cell lysates,
Lane 7: MOLT4 whole cell lysates,
Lane 8: HEL whole cell lysates,
Lane 9: rat brain tissue lysates,
Lane 10: rat thymus tissue lysates,
Lane 11: mouse brain tissue lysates,
Lane 12: mouse thymus tissue lysates,
Lane 13: Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody (BA0653) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF2 at approximately 65-75 kDa. The expected band size for ATF2 is at 55 kDa.
all(4)
Figure 2. IHC analysis of ATF2 using anti-ATF2 antibody (BA0653).
ATF2 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATF2 Antibody (BA0653) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(4)
Figure 3. IHC analysis of ATF2 using anti-ATF2 antibody (BA0653).
ATF2 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATF2 Antibody (BA0653) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(4)
Figure 4. IHC analysis of ATF2 using anti-ATF2 antibody (BA0653).
ATF2 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATF2 Antibody (BA0653) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-ATF2 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

ATF2; CRE BP1; CREB 2; CREB2; CREBP1; HB16; TREB7

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ATF2

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ATF2 recombinant protein (Position: E93-E450). Human ATF2 shares 99% amino acid (aa) sequence identity with both mouse and rat ATF2.

计算分子量

55 kDa

实际分子量

65-75 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ATF2, also known as Activating transcription factor 2, is a protein that in humans is encoded by the ATF2 gene. It is mapped to 2q31.1. This gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus, it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.

Uniprot ID

P15336

文献引用格式

ATF2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0653)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

activating transcription factor 2

蛋白名全称

Cyclic AMP-dependent transcription factor ATF-2

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实验步骤

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