Anti-AMPK Alpha 1/PRKAA1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB ELISA

A00994-3

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of AMPK Alpha 1/PRKAA1 using anti-AMPK Alpha 1/PRKAA1 antibody (A00994-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AMPK Alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (A00994-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Alpha 1/PRKAA1 at approximately 64 kDa. The expected band size for AMPK Alpha 1/PRKAA1 is at 64 kDa.
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产品名称

Anti-AMPK Alpha 1/PRKAA1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

AMPK; AMPK alpha; AMPK alpha 1; AMPK subunit alpha 1; AMPK1; AMPKa1; PRKAA1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

PRKAA1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human AMPK alpha 1 recombinant protein (Position: F377-R446).

计算分子量

64 kDa

实际分子量

64 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Uniprot ID

Q13131

文献引用格式

AMPK Alpha 1/PRKAA1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00994-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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