产品名称
Anti-AMPK Alpha 1/PRKAA1 (Phospho-S496) Antibody (Clone#HDH-16)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
AMPK; AMPK alpha; AMPK alpha 1; AMPK subunit alpha 1; AMPK1; AMPKa1; PRKAA1
免疫原
A synthesized peptide derived from human AMPK alpha 1
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
文献引用格式
AMPK Alpha 1/PRKAA1 (Phospho-S496) Antibody (Clone#HDH-16) (Boster Biological Technology, Wuhan, China. Catalog#BM4718)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
5'-AMP-activated protein kinase catalytic subunit alpha-1
推荐稀释比
Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:20 |