Anti-AMPK Alpha 1/PRKAA1 Antibody

筛选器： All WB FCM ELISA

• 

  Figure 1. Western blot analysis of AMPK Alpha 1/PRKAA1 using anti-AMPK Alpha 1/PRKAA1 antibody (A00994-6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Human Jurkat whole cell lysates,
  Lane 2: Human HELA whole cell lysates,
  Lane 3: Human MCF-7 whole cell lysates,
  Lane 4: Monkey COS-7 whole cell lysates,
  Lane 5: Human K562 whole cell lysates,
  Lane 6: Human HEK293 whole cell lysates,
  Lane 7: Rat brain tissue lysates,
  Lane 8: Rat heart tissue lysates,
  Lane 9: Mouse brain tissue lysates,
  Lane 10: Mouse heart tissue lysates,
  Lane 11: Mouse intestines tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AMPK Alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (A00994-6) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Alpha 1/PRKAA1 at approximately 64 kDa. The expected band size for AMPK Alpha 1/PRKAA1 is at 64 kDa.

• 

  Figure 2. Flow Cytometry analysis of SiHa cells using anti-AMPK Alpha 1/PRKAA1 antibody (A00994-6).
  Overlay histogram showing SiHa cells stained with A00994-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK Alpha 1/PRKAA1 Antibody (A00994-6) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Figure 1. Western blot analysis of AMPK Alpha 1/PRKAA1 using anti-AMPK Alpha 1/PRKAA1 antibody (A00994-6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Human Jurkat whole cell lysates,
  Lane 2: Human HELA whole cell lysates,
  Lane 3: Human MCF-7 whole cell lysates,
  Lane 4: Monkey COS-7 whole cell lysates,
  Lane 5: Human K562 whole cell lysates,
  Lane 6: Human HEK293 whole cell lysates,
  Lane 7: Rat brain tissue lysates,
  Lane 8: Rat heart tissue lysates,
  Lane 9: Mouse brain tissue lysates,
  Lane 10: Mouse heart tissue lysates,
  Lane 11: Mouse intestines tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AMPK Alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (A00994-6) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Alpha 1/PRKAA1 at approximately 64 kDa. The expected band size for AMPK Alpha 1/PRKAA1 is at 64 kDa.

• 

  Figure 2. Flow Cytometry analysis of SiHa cells using anti-AMPK Alpha 1/PRKAA1 antibody (A00994-6).
  Overlay histogram showing SiHa cells stained with A00994-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK Alpha 1/PRKAA1 Antibody (A00994-6) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

•