Figure 1. Western blot analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Mouse liver tissue lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-N-Cadherin/CDH2 antigen affinity purified polyclonal antibody (A01577-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for N-Cadherin/CDH2 at approximately 140 kDa. The expected band size for N-Cadherin/CDH2 is at 100 kDa.
Figure 2. Flow Cytometry analysis of HepG2 cells using anti-N-Cadherin/CDH2 antibody (A01577-3).
Overlay histogram showing HepG2 cells stained with A01577-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 3. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis using anti- CDH2 antibody (A01577-3). detected in paraffin-embedded section of human liver cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(7) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF) : | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Mouse liver tissue lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-N-Cadherin/CDH2 antigen affinity purified polyclonal antibody (A01577-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for N-Cadherin/CDH2 at approximately 140 kDa. The expected band size for N-Cadherin/CDH2 is at 100 kDa.
Figure 2. Flow Cytometry analysis of HepG2 cells using anti-N-Cadherin/CDH2 antibody (A01577-3).
Overlay histogram showing HepG2 cells stained with A01577-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 3. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis using anti- CDH2 antibody (A01577-3). detected in paraffin-embedded section of human liver cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Mouse liver tissue lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-N-Cadherin/CDH2 antigen affinity purified polyclonal antibody (A01577-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for N-Cadherin/CDH2 at approximately 140 kDa. The expected band size for N-Cadherin/CDH2 is at 100 kDa.
Figure 2. Flow Cytometry analysis of HepG2 cells using anti-N-Cadherin/CDH2 antibody (A01577-3).
Overlay histogram showing HepG2 cells stained with A01577-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 3. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis using anti- CDH2 antibody (A01577-3). detected in paraffin-embedded section of human liver cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
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