Anti-N-Cadherin/CDH2 Antibody (Clone#FI-3)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF

BM3921

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-N-Cadherin/CDH2 antibody (BM3921). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-N-Cadherin/CDH2 antigen affinity purified monoclonal antibody (BM3921) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for N-Cadherin/CDH2 at approximately 140 kDa. The expected band size for N-Cadherin/CDH2 is at 100 kDa.
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Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded human colon, using N Cadherin Antibody.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody.
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Immunofluorescent analysis using the Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-N-Cadherin/CDH2 Antibody (Clone#FI-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cadherin 2; CD325; CDH2; CDHN; CDw325; N cadherin; NCAD; N-cadherin; Neural cadherin

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

CDH2

克隆号

FI-3

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human N Cadherin

计算分子量

100 kDa

实际分子量

140 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Uniprot ID

P19022

文献引用格式

N-Cadherin/CDH2 Antibody (Clone#FI-3) (Boster Biological Technology, Wuhan, China. Catalog#BM3921)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Cadherin-2

实验方案>

实验步骤

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