Anti-N-Cadherin/CDH2 Antibody (Clone#OTI3B10)

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of anti-N-Cadherin/CDH2 antibody (M01577-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: rat C6 whole cell lysates,
  Lane 4: rat RH-35 whole cell lysates,
  Lane 5:mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-N-Cadherin/CDH2 antigen affinity purified monoclonal antibody (M01577-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for N-Cadherin/CDH2 at approximately 140 kDa. The expected band size for N-Cadherin/CDH2 is at 100 kDa.

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  Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01577-5)

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  Figure 3. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01577-5)

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  Figure 4. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDH2 antibody, and then analyzed by flow cytometry.

• 

  Figure 1. Western blot analysis of anti-N-Cadherin/CDH2 antibody (M01577-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: rat C6 whole cell lysates,
  Lane 4: rat RH-35 whole cell lysates,
  Lane 5:mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-N-Cadherin/CDH2 antigen affinity purified monoclonal antibody (M01577-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for N-Cadherin/CDH2 at approximately 140 kDa. The expected band size for N-Cadherin/CDH2 is at 100 kDa.

• 

  Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01577-5)

• 

  Figure 3. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01577-5)

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  Figure 4. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDH2 antibody, and then analyzed by flow cytometry.