Anti-Claudin 1/CLDN1 Antibody

Rabbit polyclonal antibody

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筛选器： All IHC ICC/IF IF

A01585-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. IHC analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody (A01585-2).
Claudin 1/CLDN1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 2. IHC analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody (A01585-2).
Claudin 1/CLDN1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody (A01585-2).
Claudin 1/CLDN1 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 4. IF analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody (A01585-2).
Claudin 1/CLDN1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Figure 5.IF analysis using anti- CLDN1 antibody (A01585-2) . detected in paraffin-embedded section of human lung cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
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产品简介>

产品名称

Anti-Claudin 1/CLDN1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

claudin 1; CLD1; CLDN1; ILVASC; SEMP1

产品类型

Polyclonal

检验物种

human

应用范围

IHC, ICC/IF, IF

基因名称

CLDN1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Claudin 1/CLDN1, identical to the related mouse and rat sequences.

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Uniprot ID

O95832

文献引用格式

Claudin 1/CLDN1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01585-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

claudin 1

蛋白名全称

Claudin-1

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实验步骤

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