Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- CLDN1 antibody (BM5140). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: Caco-2 whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
Use mouse anti- CLDN1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CLDN1 at approximately 19KD. The expected band size for CLDN1 is at 23KD.
Figure 1. Western blot analysis of anti- CLDN1 antibody (BM5140). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: Caco-2 whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
Use mouse anti- CLDN1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CLDN1 at approximately 19KD. The expected band size for CLDN1 is at 23KD.