Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of anti- Claudin 1/CLDN1 antibody (A01585-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
Use rabbit anti- Claudin 1/CLDN1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Claudin 1/CLDN1 at approximately 22KD. The expected band size for Claudin 1/CLDN1 is at 28KD.
Figure 1. Western blot analysis of anti- Claudin 1/CLDN1 antibody (A01585-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
Use rabbit anti- Claudin 1/CLDN1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Claudin 1/CLDN1 at approximately 22KD. The expected band size for Claudin 1/CLDN1 is at 28KD.