Western blot analysis of anti- Cytokeratin 1/KRT1 antibody (A01639). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human Hela whole cell lysates.
Use rabbit anti- Cytokeratin 1/KRT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Cytokeratin 1/KRT1 at approximately 66KD. The expected band size for Cytokeratin 1/KRT1 is at 66KD.
IHC analysis of Cytokeratin 1/KRT1 using anti-Cytokeratin 1/KRT1 antibody (A01639).
Cytokeratin 1/KRT1 was detected in a paraffin-embedded section of human skin cancer tissue. The tissue section was incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- Cytokeratin 1/KRT1 antibody (A01639). detected in paraffin-embedded section of human skin cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).
all(5) 
Flow Cytometry analysis of Jurkat cells using anti-Cytokeratin 1/KRT1 antibody (A01639).
Overlay histogram showing Jurkat cells stained with A01639 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti- Cytokeratin 1/KRT1 antibody (A01639). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human Hela whole cell lysates.
Use rabbit anti- Cytokeratin 1/KRT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Cytokeratin 1/KRT1 at approximately 66KD. The expected band size for Cytokeratin 1/KRT1 is at 66KD.
IHC analysis of Cytokeratin 1/KRT1 using anti-Cytokeratin 1/KRT1 antibody (A01639).
Cytokeratin 1/KRT1 was detected in a paraffin-embedded section of human skin cancer tissue. The tissue section was incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- Cytokeratin 1/KRT1 antibody (A01639). detected in paraffin-embedded section of human skin cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).
Flow Cytometry analysis of Jurkat cells using anti-Cytokeratin 1/KRT1 antibody (A01639).
Overlay histogram showing Jurkat cells stained with A01639 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of anti- Cytokeratin 1/KRT1 antibody (A01639). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human Hela whole cell lysates.
Use rabbit anti- Cytokeratin 1/KRT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Cytokeratin 1/KRT1 at approximately 66KD. The expected band size for Cytokeratin 1/KRT1 is at 66KD.
IHC analysis of Cytokeratin 1/KRT1 using anti-Cytokeratin 1/KRT1 antibody (A01639).
Cytokeratin 1/KRT1 was detected in a paraffin-embedded section of human skin cancer tissue. The tissue section was incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- Cytokeratin 1/KRT1 antibody (A01639). detected in paraffin-embedded section of human skin cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).
Flow Cytometry analysis of Jurkat cells using anti-Cytokeratin 1/KRT1 antibody (A01639).
Overlay histogram showing Jurkat cells stained with A01639 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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