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Anti-Pan Cytokeratin Antibody (Clone#C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

Mouse monoclonal antibody

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筛选器: All WB IHC

BM0034

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-Pan KRT1 antibody (BM0034). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human NCL-H460 whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human A431 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Pan KRT1 antigen affinity purified monoclonal antibody (BM0034) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Pan KRT1 at approximately 45-60 kDa. The expected band size for Pan KRT1 is at 66 kDa.

    all(2)
  • IHC analysis of Pan KRT1 using anti-Pan KRT1 antibody (BM0034).
    Pan KRT1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-Pan Cytokeratin Antibody (Clone#C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
67 kDa cytokeratin; CK 1; CK1; Cytokeratin 1; keratin 1; KRT1
产品类型
Monoclonal
检验物种
human, rat
应用范围
WB, IHC
基因名称
KRT1
克隆号
C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2
宿主
Mouse
抗体亚型
IgG2a/ IgG1
免疫原
mixture of several monoclonal cytokeratin clones.
计算分子量
66 kDa
实际分子量
66 kDa
成分
200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
200ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin(mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.
Uniprot ID
P04264  
文献引用格式
Pan Cytokeratin Antibody (Clone#C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) (Boster Biological Technology, Wuhan, China. Catalog#BM0034)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Pan Cytokeratin Antibody (Clone#C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

    筛选器: All WB IHC

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