Anti-Nephrin/NPHS1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A01991

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Nephrin/NPHS1 using anti-Nephrin/NPHS1 antibody (A01991). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Nephrin/NPHS1 antigen affinity purified polyclonal antibody (A01991) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Nephrin/NPHS1 at approximately 185 kDa. The expected band size for Nephrin/NPHS1 is at 135 kDa.
all(3)
IHC analysis of Nephrin/NPHS1 using anti-Nephrin/NPHS1 antibody (A01991).
Nephrin/NPHS1 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Nephrin/NPHS1 Antibody (A01991) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of Nephrin/NPHS1 using anti-Nephrin/NPHS1 antibody (A01991).
Nephrin/NPHS1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Nephrin/NPHS1 Antibody (A01991) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-Nephrin/NPHS1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NPHS1adhesion molecule; nephrin; CNF; NPHN; nephrin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

NPHS1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Nephrin recombinant protein (Position: V1084-V1241). Human Nephrin shares 79.9% and 75.3% amino acid (aa) sequence identity with mouse and rat Nephrin, respectively.

计算分子量

135 kDa

实际分子量

185 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

NPHS1, also called Nephrin, is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. It gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, orproteinuria. NPHS1 is also required for cardiovascular development.

Uniprot ID

O60500

RRID

AB_3081508

文献引用格式

Nephrin/NPHS1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01991)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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