Anti-Nephrin/NPHS1 Antibody (Clone#OTI1G6)

Mouse monoclonal antibody

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M01991-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPHS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPHS1.(1:1000)
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产品名称

Anti-Nephrin/NPHS1 Antibody (Clone#OTI1G6)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NPHS1adhesion molecule; nephrin; CNF; NPHN; nephrin

产品类型

Monoclonal

检验物种

human

应用范围

基因名称

NPHS1

克隆号

OTI1G6

宿主

Mouse

抗体亚型

IgG2b

免疫原

Human recombinant protein fragment corresponding to amino acids 1077-1241 of human NPHS1 (NP_004637) produced in E.coli.

计算分子量

135 kDa

实际分子量

185 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.

背景资料

NPHS1, also called Nephrin, it is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, orproteinuria. NPHS1 is also required for cardiovascular development.

Uniprot ID

O60500

文献引用格式

Nephrin/NPHS1 Antibody (Clone#OTI1G6) (Boster Biological Technology, Wuhan, China. Catalog#M01991-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nephrosis 1, congenital, Finnish type (nephrin)

蛋白名全称

Nephrin

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实验步骤

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