Anti-Nephrin/NPHS1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA1669

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Nephrin/NPHS1 using anti-Nephrin/NPHS1 antibody (BA1669). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Nephrin/NPHS1 antigen affinity purified polyclonal antibody (BA1669) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Nephrin/NPHS1 at approximately 135 kDa. The expected band size for Nephrin/NPHS1 is at 135 kDa.
all(2)
IHC analysis of Nephrin/NPHS1 using anti-Nephrin/NPHS1 antibody (BA1669).
Nephrin/NPHS1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Nephrin/NPHS1 Antibody (BA1669) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-Nephrin/NPHS1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NPHS1adhesion molecule; nephrin; CNF; NPHN; nephrin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

NPHS1

宿主

Rabbit

抗体亚型

IgG

免疫原

Polypeptide

计算分子量

135 kDa

实际分子量

135 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Uniprot ID

O60500

文献引用格式

Nephrin/NPHS1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1669)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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