Figure 1. Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody (A02038-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Jun/JUN at approximately 36-48 kDa. The expected band size for c-Jun/JUN is at 36 kDa.
Figure 2. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody (A02038-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Jun/JUN at approximately 36-48 kDa. The expected band size for c-Jun/JUN is at 36 kDa.
Figure 2. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 1. Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody (A02038-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Jun/JUN at approximately 36-48 kDa. The expected band size for c-Jun/JUN is at 36 kDa.
Figure 2. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in a paraffin-embedded section of human spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2).
c-Jun/JUN was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-c-Jun/JUN Antibody (A02038-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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